Zebrafish Editing

CRISPR and Tol2 - Knudra Has the Expertise to Provide the Transgenics You Need.

With RediSERVICE, Knudra provides a genome engineering service to fit a variety of project types.

  • CRISPR - obtain gene knock-outs (KOs) or knock-ins (KIs) in zebrafish at the native loci.
  • Tol2 - obtain gene knock-ins (KIs) in zebrafish with expression at random insertion loci.
Our RediSERVICE platform uses our depth of experience in other model organisms to bring you reliable CRISPR/Cas9 transgenics in zebrafish. For instance, our genome engineering expertise has been used extensively in the creation of GFP and mCherry knock-ins for zebrafish clients. Similarly, clients have used our CRISPR expertise to get creation of targeted point mutations at native loci for modeling human disease. In addition to CRISPR, we also offer transposon-mediated gene insertion services utilizing the Tol2 methods. Use of Tol2 cargo insertions is the method of choice for building large and complex constructs for ectopic and tissue-specific expression. Get started on a project today by exploring the links above for how our RediSERVICE platform can fit your needs.