C. elegans CRISPR Services

Knudra specializes in custom CRISPR services in C. elegans. Using a variety of methods, Knudra has designed and delivered over 1,500 transgenic lines to meet the research needs of our clients. Below are several types of CRISPR services offer by Knudra. 

TrueDEL - Full gene deletion (up to 12kB)

A trueDEL CRISPR knock-out (KO) project is designed to delete the full coding sequence of any desired gene. Timelines: RediMIX - 1 week; Full-Build - 4 week.

quote for trueDEL

 

Tagging - GFP or other tag inserted

A Tagging CRISPR knock-in (KI) project is designed to insert either a fluorescent tag (typically GFP or mCherry), or to insert a pull-down tag (typically 3xFLAG, HA, or TAP). Timelines: RediMIX - 3 week; Full-Build - 6 week.

quote for Tagging

 

PointMUTATION - Swap of amino acid content

A pointMUTATION CRISPR knock-in (KI) project is designed to change an amino aicd or small region in a gene. Timelines: RediMIX - 1 week; Full-Build - 4 week.

quote for pointMUTATION

 

ExonDEL - Exon deletion

A exonDEL CRISPR project is designed to delete a section of coding sequence of any desired gene. Typically a conserved exon, or group of exons, is deleted and may result in gene function knock-out (KO). Timelines: RediMIX - 1 week; Full-Build - 4 week.

quote for exonDEL

 

RandomINDEL - Non-specific mutant generation

A randomINDEL CRISPR project is designed to randomily insert or delete content. No donor homology used. Highly efficient but imprecise indel project that may or may not result in a gene knock-out (KO). Timelines: Timelines: RediMIX - 1 week; Full-Build - 4 week.

quote for randomINDEL

 

GeneREPLACEMEMT - Swap out of entire gene

A geneREPLACEMEMT CRISPR knock-in (KI) project is designed to remove and replace a gene with a different gene content. Timelines: RediMIX - 5 week; Full-Build - 8 week.

quote for geneREPLACEMEMT

 

Other method ideas? - Contact us

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CRISPR- trueDEL

 

CRISPR project is designed to delete the full coding sequence of any desired gene. 

 
C. elegans CRISPR trueDEL

CRISPR - exonDEL

 

CRISPR project is designed for a precision deletion of a segment of a gene. 

 
C. elegans CRISPR exonDEL

CRISPR - randomINDEL

 

CRISPR project is designed to generate random mutations occurring frequently as small indels.

 
C. elegans CRISPR randomINDEL

CRISPR - Tagging

 

CRISPR project is designed to tag either termini of your desired gene.  Tagging examples are GFP mCherry, 3xFLAG, HA, TAP, etc. 

 
C. elegans CRISPR Tagging

CRISPR - pointMUTATION

 

CRISPR project is designed to change a amino acid or other small region of coding. 

 
C. elegans CRISPR pointMUTATION

CRISPR - geneREPLACEMENT

 

CRISPR project designed to remove a native gene and replace with a transgene.

 
C. elegans CRISPR geneREPLACEMENT

C. elegans Transgenics Question

 

Let us know if you need more information about how our transgenics can meet your needs.

 
C. elegans Transgenics Question

Humanized Transgenics

 
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Contact us to see how we can create the humanized models you need for your research.