C. elegans CRISPR Services
Knudra specializes in custom CRISPR services in C. elegans. Using a variety of methods, Knudra has designed and delivered over 1,500 transgenic lines to meet the research needs of our clients. Below are several types of CRISPR services offer by Knudra.
Tagging - GFP or other tag inserted
A Tagging CRISPR knock-in (KI) project is designed to insert either a fluorescent tag (typically GFP or mCherry), or to insert a pull-down tag (typically 3xFLAG, HA, or TAP).quote for Tagging
ExonDEL - Exon deletion
A exonDEL CRISPR project is designed to delete a section of coding sequence of any desired gene. Typically a conserved exon, or group of exons, is deleted and may result in gene function knock-out (KO).quote for exonDEL
RandomINDEL - Non-specific mutant generation
A randomINDEL CRISPR project is designed to randomily insert or delete content. No donor homology used. Highly efficient but imprecise indel project that may or may not result in a gene knock-out (KO).quote for randomINDEL