C. elegans RediMIX

Knudra designs and makes injection mix - You inject and find transgenics.

RediMIX logo (2).png
  • Fast - inject and isolate transgenic line in as soon as 10 days
  •  
  • Easy - 3 steps to transgenics
    • step 1 - Request a specific project type
    • step 2 - Knudra designs and makes injection mix
    • step 3 - You inject animals and screen at your liesure

Prices Starting at $495

Optional protocol, services and screening reagents available upon request.

Project Specificity

rm-RediMIXMake Indels - Quickly produce loss-of-function alleles by random mutagenesis.

pd-RediMIXObtain Precision Deletion - Knock out all coding elements so there is no need to wonder about partial function anymore.

pm-RediMIXInsert Point Mutations - Model human disease mutations to bring higher relevance to your work.

tn-RediMIXTag Native Locus - Put in GFP, or any other tag, at any position in the genome.

fx-RediMIXMake Floxed Alleles - Put in loxP, or similar sites, so that you can make induced conditionals.

 

have a custom mix idea? email us

rm-RediMIX - Random Deletion

Knudra designs and makes mix - you inject and find transgenic.

Mix Includes:

  • sgRNA
    • expertly selected to have high efficiency for guiding cutting and reducing off-target mutations
    •  
  • Cas9 protein
  •  
PRICE: $495

Addons:
- in vivo testing of the sgRNAs - $1,360
- PCR screening protocol and reagents kit - $650
 

pm-RediMIX - Point Mutations

Knudra designs and makes mix - you inject and find transgenic.

Mix includes:

  • sgRNAs
    • expertly selected to have high efficiency for guiding cutting and reducing off-target mutations
    •  
  • donor homology
    • ssDNA expertly designed to prevent recutting and promote complete edit
    •  
  • Cas9 protein
  •  
  • dpy-10 co-CRISPR
    • to monitor injection efficiency and help select edited animals
    •  
PRICE: $995

Addons:
- in vivo testing of the sgRNAs - $1,360
- PCR screening protocol and reagents kit - $650
 

pd-RediMIX - Precision Deletion

Knudra designs and makes mix - you inject and find transgenic.

Mix includes:

  • sgRNAs
    • expertly selected to have high efficiency for guiding cutting and reducing off-target mutations
    •  

  • donor homology
    • ssDNA expertly designed to prevent recutting and promote complete edit
     

  • Cas9 protein
  •  

  • dpy-10 co-CRISPR
  • to monitor injection efficiency and help select edited animals
     
PRICE: $995

Addons:
- in vivo testing of the sgRNAs - $1,360
- PCR screening protocol and reagents kit - $650
 

tn-RediMIX - Tag Native Locus

Knudra designs and makes mix - you inject and find transgenic.

Mix includes:

  • sgRNAs
    • expertly selected to have high efficiency for guiding cutting and reduced off-target mutations
    •  
  • donor homology
    • DNA expertly designed to prevent recutting and promote complete edit (price variable)
    •  
  • Cas9 protein
     

  • dpy-10 co-CRISPR
  • to monitor injection efficiency and help select edited animals
PRICE: $1100-1900

Addons:
- in vivo testing of the sgRNAs - $1,360
- PCR screening protocol and reagents kit - $650
 

fx-RediMIX - Floxed Conditional Knockout

Knudra designs and makes mix - you inject and find transgenic.

Mix includes:

  • sgRNAs
    • expertly selected to have high efficiency for guiding cutting and reduced off-target mutations
    •  
  • donor homology
    • DNA expertly designed to prevent recutting and promote complete insertion of floxed exon (price variable)
    •  

  • Cas9 protein
  •  

  • dpy-10 co-CRISPR
    • to monitor injection efficiency and help select edited animals
     
PRICE: $1100-1700

Addons:
- in vivo testing of the sgRNAs - $1,360
- PCR screening protocol and reagents kit - $650