RediMODEL transgenics in C. elegans
PTEN - tumor suppressor
- Function: PTEN is a phosphatase and tensin homolog involved in ATK/PKB signaling.
- Disease: Genetic defects in PTEN have been found to be highly associated with carcinoma tumors.
RediMODEL Kits - a series of humanized animal models specifically engineered for easy measurement of phenotype defects caused by clinically-relevant disease mutations.
PTEN RediMODEL KO: Generated in the C. elegans homolog daf-18 with partial deletion of the coding sequence to create a knock-out (KO) strain. Loss of function from daf-18 partial deletion leads to deletion of C2 domain and tail needed for proper plasma membrane localization. To create a fast phenotype screen, a synthetic interaction is used to create an easy to score egg laying defect. The synthetic background is available for preorder as gene knock-out.
PTEN RediMODEL KI: Generated in the C. elegans homolog daf-18 with human gene insertion to create a knock-in (KI) strain. Entire coding of daf-18 is replaced with wild-type PTEN coding sequence. A PTEN gene replacement is available for preorder in a synthetic background as a humanized wt line.
PTEN RediMODEL CV: Generated in the C. elegans homolog daf-18 with human gene insertion containing a clinical variant allele to create a clinical-variant (CV) strain. Entire coding of daf-18 is replaced with PTEN coding sequence containing amino-acid change polymorphism that has been observed in patient populations. Upon request, a PTEN line with client-specific polymorphism is made in a synthetic background as a clinical variant allele.