RediMODEL transgenics in C. elegans

PTEN - tumor suppressor

GENE DESCRIPTION:

  • Function: PTEN is a phosphatase and tensin homolog involved in ATK/PKB signaling.
  • Disease: Genetic defects in PTEN have been found to be highly associated with carcinoma tumors.
 

Products:

RediMODEL Kits -  a series of humanized animal models specifically engineered for easy measurement of phenotype defects caused by clinically-relevant disease mutations.   

rediMODEL-KO

PTEN RediMODEL KO: Generated in the C. elegans homolog daf-18 with partial deletion of the coding sequence to create a knock-out (KO) strain. Loss of function from daf-18 partial deletion leads to deletion of C2 domain and tail needed for proper plasma membrane localization. To create a fast phenotype screen, a synthetic interaction is used to create an easy to score egg laying defect. The synthetic background is available for preorder as gene knock-out.

 

PTEN RediMODEL KI: Generated in the C. elegans homolog daf-18 with human gene insertion to create a knock-in (KI) strain. Entire coding of daf-18 is replaced with wild-type PTEN coding sequence. A PTEN gene replacement is available for preorder in a synthetic background as a humanized wt line.

 

PTEN RediMODEL CV: Generated in the C. elegans homolog daf-18 with human gene insertion containing a clinical variant allele to create a clinical-variant (CV) strain. Entire coding of daf-18 is replaced with PTEN coding sequence containing amino-acid change polymorphism that has been observed in patient populations. Upon request, a PTEN line with client-specific polymorphism is made in a synthetic background as a clinical variant allele.

 

RediMODEL knock-out in C. elegans | $845 each kit Buy Now

PTEN - tumor suppressor

DESCRIPTION:

The PTEN gene is a phosphatase and tensin homolog. Enzymatically it is phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase that acts as tumor suppressor by negatively regulating AKT/PKB signaling pathway (other names: BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, PTENbeta)

DISEASES:

Carcinoma, Autistic Disorder, Acute Megakaryocytic Leukemias, Adenocarcinoma, Adenoid Cystic Carcinoma, Anisometropia, Asthma, Bannayan-Riley-Ruvalcaba Syndrome, Craniofacial Abnormalities, Dermatologic disorders, Developmental Disabilities, Disease Progression, Dysplastic gangliocytoma, Endometrial Carcinoma, Endometrial Hyperplasia, Endometrial Neoplasms, Familial meningioma, Focular thyroid cancer, Glioma, Hemangioma, Hereditary Breast and Ovarian Cancer, Hypertensive disease, IGA Glomerulonephritis, Insulin Resistance, Intellectual Disability, Juvenile polyposis syndrome, Language Development Disorders, LEOPARD Syndrome, leukemia, Lhermitte-Duclos disease, Lipoma, Lipomatosis, Long QT Syndrome, Lung Neoplasms, Lymphoma, Malignant neoplasm of prostate, Mammary Neoplasms, Mediastinal Germ Cell Tumor, melanoma, Meningioma, Motor Skills Disorders, Multiple Hamartoma Syndrome, Myocardial Reperfusion Injury, Nasopharyngeal carcinoma, Neoplasm Invasiveness, Neoplasm Metastasis, ovarian neoplasm, Pancreatic Neoplasm, Polydactyly, Prostatic Neoplasms, Proteus Syndrome, PTEN Hamartoma Tumor Syndrome, Renal Cell Carcinoma, Reperfusion Injury, Sezary Syndrome, Small cell carcinoma of lung, Squamous cell carcinoma.*

* All disease associations scored above a 0.1 threshold from disgenet.com

Protein domain - slc6a3 dat-1.png

HOMOLOGY:

Human Gene PTEN Alignment         
C. elegans Gene daf-18 align
Identity 31%
Similarity 46%

KNOCK-OUT of C. elegans gene:

daf-18 is the homologous gene to PTEN. The daf-18 gene functions to control germ cell replication by ATK/PKB signaling. When daf-18 is expressed and active, it acts as a phosphatidylinositol phosphatase enable progression into dauer life-state.  Since loss of function alleles in daf-18 have a phenotype that is late in onset, the daf-18 alleles are combined with unc-74 to create a synthetic strain showing 15x decreased brood size. Because gene deletions are not currently available in daf-18, a full deletion of the daf-18 locus is used instead. The full deletion allele is offered as the PTEN RediMODEL-KO strain as a 4x outcrossed allele in the unc-74 background. The KO strain is confirmed by PCR and shipped in the RediMODEL kit format. Included in the kit is N2 wild-type strain as a reference control.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of PTEN RediMODEL-KO and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for the researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KO line is sequence verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set used to verify the RediMODEL kit.



amplicon size
wild type 500 bp
PTEN RediMODEL-KO 900 bp
 

Phenotype data:

Bio Assay

The RediMODEL-KO line's activity is best followed by monitoring changes in brood size using the Brood Sizing Protocol (P006), a significant 15x difference difference in brood size can be expected.

Brood Size Protocol (P006)

strain normal brood size
N2 Standard Strain 100%
PTEN RediMODEL-KO 5%

Discover PTEN RediMODEL-KO Kit: | $845 each kit Buy Now

Discover RediMODEL-KO Kit: | $845 each kit

Inquire on PTEN RediMODEL-KO

Please provide contact info and a company representative will contact you soon with preorder invoicing details.

PTEN rediMODEL-KO inquiry

RediMODEL knock-in of human gene replacement in C. elegans | $845 each kit Buy Now

PTEN - tumor suppressor

DESCRIPTION:

The PTEN gene is a phosphatase and tensin homolog. Enzymatically it is phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase that acts as tumor suppressor by negatively regulating AKT/PKB signaling pathway (other names: BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, PTENbeta)

DISEASES:

Carcinoma, Autistic Disorder, Acute Megakaryocytic Leukemias, Adenocarcinoma, Adenoid Cystic Carcinoma, Anisometropia, Asthma, Bannayan-Riley-Ruvalcaba Syndrome, Craniofacial Abnormalities, Dermatologic disorders, Developmental Disabilities, Disease Progression, Dysplastic gangliocytoma, Endometrial Carcinoma, Endometrial Hyperplasia, Endometrial Neoplasms, Familial meningioma, Focular thyroid cancer, Glioma, Hemangioma, Hereditary Breast and Ovarian Cancer, Hypertensive disease, IGA Glomerulonephritis, Insulin Resistance, Intellectual Disability, Juvenile polyposis syndrome, Language Development Disorders, LEOPARD Syndrome, leukemia, Lhermitte-Duclos disease, Lipoma, Lipomatosis, Long QT Syndrome, Lung Neoplasms, Lymphoma, Malignant neoplasm of prostate, Mammary Neoplasms, Mediastinal Germ Cell Tumor, melanoma, Meningioma, Motor Skills Disorders, Multiple Hamartoma Syndrome, Myocardial Reperfusion Injury, Nasopharyngeal carcinoma, Neoplasm Invasiveness, Neoplasm Metastasis, ovarian neoplasm, Pancreatic Neoplasm, Polydactyly, Prostatic Neoplasms, Proteus Syndrome, PTEN Hamartoma Tumor Syndrome, Renal Cell Carcinoma, Reperfusion Injury, Sezary Syndrome, Small cell carcinoma of lung, Squamous cell carcinoma.*

* All disease associations scored above a 0.1 threshold from disgenet.com

Protein domain - slc6a3 dat-1.png

HOMOLOGY:

Human Gene PTEN Alignment         
C. elegans Gene daf-18 align
Identity 31%
Similarity 46%
RediMODEL-KI logo sml.png

HUMANIZED C. elegans:

daf-18 is the homologous gene to PTEN. The daf-18 gene functions to control germ cell replication by ATK/PKB signaling. When daf-18 is expressed and active, it acts as a phosphatidylinositol phosphatase enable progression into dauer life-state.  Deletion mutations in daf-18  as PTEN RediMODEL-KO in unc-74(e963) background cause a 10x decrease in brood size. To create a humanized worm, PTEN gene is swapped for the daf-18 coding segment.   Installation of PTEN in replacement of daf-18 causes restoration of normal egg laying phenotype and is offered as PTEN RediMODEL-KI in the unc-74 background. The KI  strain is confirmed by PCR and shipped in the RediMODEL kit format. Included in the kit is PTEN RediMODEL-KO as a reference control.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of PTEN RediMODEL-KI and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for the researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KI line is sequence verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set used to verify the RediMODEL kit.

amplicon size
wild type 500 bp
PTEN RediMODEL-KI 900 bp
 

Phenotype data:

Bio Assay

The RediMODEL-KI line's activity is best followed by monitoring changes in brood size using the Brood Sizing Protocol (P006), a significant 15x difference difference in brood size can be expected.

Brood Size Protocol (P006)

strain normal brood size
PTEN RediMODEL-KO 5%
PTEN RediMODEL-KI 100%

Discover PTEN RediMODEL-KI Kit: | $845 each kit Buy Now

Discover RediMODEL-KI Kit: | $845 each kit

Inquire on PTEN RediMODEL-KI

Please provide contact info and a company representative will contact you soon with preorder details.

PTEN rediMODEL-KI inquiry

RediMODEL clinical variant in C. elegans | $6695 each kit Buy Now

PTEN - tumor suppressor

DESCRIPTION:

The PTEN gene is a phosphatase and tensin homolog. Enzymatically it is phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase that acts as tumor suppressor by negatively regulating AKT/PKB signaling pathway (other names: BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, PTENbeta)

DISEASES:

Carcinoma, Autistic Disorder, Acute Megakaryocytic Leukemias, Adenocarcinoma, Adenoid Cystic Carcinoma, Anisometropia, Asthma, Bannayan-Riley-Ruvalcaba Syndrome, Craniofacial Abnormalities, Dermatologic disorders, Developmental Disabilities, Disease Progression, Dysplastic gangliocytoma, Endometrial Carcinoma, Endometrial Hyperplasia, Endometrial Neoplasms, Familial meningioma, Focular thyroid cancer, Glioma, Hemangioma, Hereditary Breast and Ovarian Cancer, Hypertensive disease, IGA Glomerulonephritis, Insulin Resistance, Intellectual Disability, Juvenile polyposis syndrome, Language Development Disorders, LEOPARD Syndrome, leukemia, Lhermitte-Duclos disease, Lipoma, Lipomatosis, Long QT Syndrome, Lung Neoplasms, Lymphoma, Malignant neoplasm of prostate, Mammary Neoplasms, Mediastinal Germ Cell Tumor, melanoma, Meningioma, Motor Skills Disorders, Multiple Hamartoma Syndrome, Myocardial Reperfusion Injury, Nasopharyngeal carcinoma, Neoplasm Invasiveness, Neoplasm Metastasis, ovarian neoplasm, Pancreatic Neoplasm, Polydactyly, Prostatic Neoplasms, Proteus Syndrome, PTEN Hamartoma Tumor Syndrome, Renal Cell Carcinoma, Reperfusion Injury, Sezary Syndrome, Small cell carcinoma of lung, Squamous cell carcinoma.*

* All disease associations scored above a 0.1 threshold from disgenet.com

Protein domain - slc6a3 dat-1.png

HOMOLOGY:

Human Gene PTEN Alignment         
C. elegans Gene daf-18 align
Identity 31%
Similarity 46%
rediMODEL-KO

CLINICAL VARIANT C. elegans:

daf-18 is the homologous gene to PTEN. The daf-18 gene functions to control germ cell replication by ATK/PKB signaling. When daf-18 is expressed and active, it acts as a phosphatidylinositol phosphatase enable progression into dauer life-state.  Deletion mutations in daf-18  as PTEN RediMODEL-KO in unc-74(e963) background cause a 10x decrease in brood size.  Installation of PTEN in replacement of daf-18 results in the PTEN RediMODEL-KI control strain and cause restoration of normal egg laying phenotype.  A client-specified clinical variant is installed into the PTEN sequence of the wt-control strain in the unc-74 background.  The result is a PTEN RediMODEL-CV strain whose relative phenotypic consequence can be rapidly measured. The CV strain is confirmed by PCR and shipped in the RediMODEL kit format. Included in the kit are PTEN RediMODEL-KO and PTEN RediMODEL-KI as reference controls.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of XXXXX RediMODEL-CV and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for the researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-CV line is sequence verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set used to verify the RediMODEL kit.



amplicon size
wild type 500 bp
PTEN RediMODEL-CV 900 bp
 

Phenotype data:

Bio Assay

The RediMODEL-CV line's activity is best followed by monitoring changes in brood size using the Brood Sizing Protocol (P006), a significant 15x difference difference in brood size can be expected.

Brood Size Protocol (P006)

strain normal brood size
PTEN RediMODEL-KO 5%
PTEN RediMODEL-CV ???%
PTEN RediMODEL-KI 100%

Discover PTEN RediMODEL-CV Kit: | $6695 each kit Buy Now

Discover RediMODEL-CV Kit: | $6695 each kit

Inquire on PTEN RediMODEL-CV

Please provide contact info and a company representative will contact you soon with design and invoicing details.

PTEN rediMODEL-CV inquiry

Alignment of PTEN to daf-18

Alignment Length Identity Similarity Gaps
432 31% 46% 20%

Lets us know if you want to model clinical variants of PTEN in the native daf-18 gene.

RediMODEL-KInative - daf-18 with human point mutations