Accelerate Your Science
Knudra has developed several optimized plasmids to help you achieve your research goals. All products features on this page have been rigorously tested and are routinely used in our TavaServ products - our industry-leading platform for custom production of transgenics.
sqt-1 marker plasmid (pNU1228)
(100ng/ul, 15 ul volume)
The sqt-1 plasmid encodes for a cuticle collagen expression in C elegans. Composition is sqt-1p::SQT-1(genomic)::sqt-1utr. A single point mutation (e1350) within the gene produces a morphological phenotype similar to Rol due to an improper cuticle formation. Expression of SQT-1 is seen during L2, L3, and L4 larvae and adult stages, but not L1 or dauer larvae.
Suggested use: This plasmid is suggested as a co-marker of injection efficiency in C. elegans and other nematodes. Successful injections will display a Rol phenotype in L2-L4 larvae and adult worms. In the injection mix, a final concentration is commonly used at 10 ng/ul in a 100ng/ul total DNA. The reagent is supplied as a 10X stock.
myo-2::GFP marker plasmid (pNU675)
(15ng/uL, 15uL volume)
The myo-2::GFP construct expresses GFP in the pharynx in C. elegans. Composition is myo-2p::eGFP(3x-syntrons)::tbb-2utr. The myo-2 promoter drives GFP exclusively in pharynx of the animal.
Suggested use: This plasmid can be used as as a co-marker of injection efficiency in C. elegans or other similar nematodes. Successful injections will display a bright green pharynx. In the injection mix, a final concentration of 1.5 ng/ul is commonly used in a 100ng/ul total DNA. The reagent is supplied as a 10X stock.
hygR selection plasmid (pNU1254)
(100ng/ul, 15 ul volume)
The HygR plasmid encodes for broad expression of hygromycin B phosphotransferase (hpt) in C. elegans tissues. Composition is rps-0p::HPT::unc-54utr. Sufficient accumulation of expression of HPT occurs at 24 hrs post-injection. Resistant worms will be indicated by survival after as soon as 2 days after exposure to Hygromycin B (0.25mg/ml). Easiest screen occurs 5th day post hygromycin exposure where only resistant animals are present.
Suggested use: This HygR plasmid is a good selection tool for use in C. elegans or other similar nematodes. Successful injections will be resistant to Hygromycin B (0.25mg/ml) in plates. Addition of HygR plasmid into an injection mix is commonly used at a final concentration of 10 ng/ul in a 100ng/ul total DNA. The reagent is supplied as a 10X stock.