RediMODEL transgenics in C. elegans

HTT - huntingtin

GENE DESCRIPTION:

  • Function: HTT is thought to contribute to normal development.
  • Disease: Genetic defects causing disease are primarily gain of function mutations from poly-nucleotide expansions coding for long stretches of poly-glutamine (poly-Q).
 

Products:

RediMODEL Kits -  a series of humanized animal models specifically engineered for easy measurement of phenotype defects caused by clinically-relevant disease mutations.   

rediMODEL-KO

HTT RediMODEL KO: Generated in the C. elegans homolog F21G4.6 as a large deletion allele leading to over 100% deletion in the coding sequence. The F21G4.6 deletion knock-out (KO) results in loss of expression of in F21G4.6. A major phenotype readout for KO line has yet to be observed. The all-coding deletion mutant is currently available as gene knock-out.

 

HTT RediMODEL KI: Generated in the C. elegans homolog F21G4.6 with human gene insertion to create a knock-in (KI) strain. Entire coding of F21G4.6 is replaced with wild-type HTT coding sequence. An HTT gene replacement is currently available for preorder as a humanized wt line.

 

HTT RediMODEL CV: Generated in the C. elegans homolog F21G4.6 with human gene insertion containing a clinical variant allele to create a clincal variant (CV) strain. Entire coding of F21G4.6 is replaced with HTT coding sequence containing amino-acid change polymorphism that has been observed in patient populations. Upon request, a HTT line with client-specific polymorphism is made as a clinical variant allele.

 

RediMODEL knock-out in C. elegans | $845 each kit Buy Now

HTT - huntingtin

DESCRIPTION:

Huntington's disease is a neurodegenerative disorder characterized by loss of striatal neurons due to trinucleotide expansion translating into long tracks of poly-glutamine repeat. poly-Q tracks in excess of 40 have been described as pathological.

DISEASES:

Huntington's Disease, Movement Disorders, Juvenile Huntington's Disease, Cadmium poisoning, Manganese Poisoning.*

* All disease associations scored above a 0.1 threshold from disgenet.com

HOMOLOGY

Human Gene HTT Alignment         
C. elegans Gene F21G4.6 align
Identity 18%
Similarity 34%

KNOCK-OUT of C. elegans gene:

F21G4.6 is the homolog gene to HTT. The F21G4.6 gene is highly expressed in germline and neuronal tissues. Partial deletion mutations in F21G4.6 have yet to demonstrate loss-of-function phenotype. To enable assessment of the entire gene, a knock-out (KO) allele has been made eliminating all coding regions of the F21G4.6 gene. To decrease the risk of background mutations, the KO strain is 4x outcrossed. The resulting outcrossed strain containing the full deletion allele is offered as the HTT RediMODEL-KO strain. The KO strain is confirmed by PCR and shipped in the RediMODEL kit format. Included in each kit is N2 wild-type strain as a reference control.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of HTT RediMODEL-KO and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for the researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KO line is sequence verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set used to verify the RediMODEL kit.



amplicon size
wild type 500 bp
HTT RediMODEL-KO 900 bp
 

Phenotype data:

Bio Assay

Conditions for the RediMODEL-KO line to yield a phenotype have not yet been determined.


strain activity
N2 wild type 100%
HTT RediMODEL-KO ??%

Discover HTT RediMODEL-KO Kit: | $845 each kit Buy Now

Discover RediMODEL-KO Kit: | $845 each kit

Order HTT RediMODEL-KO

Please provide contact info and a company representative will contact you soon with invoicing details.

HTT rediMODEL-KO inquiry

RediMODEL knock-in of human gene replacement in C. elegans | $845 each kit Buy Now

HTT - huntingtin

DESCRIPTION:

Huntington's disease is a neurodegenerative disorder characterized by loss of striatal neurons due to trinucleotide expansion translating into long tracks of poly-glutamine repeat. poly-Q tracks in excess of 40 have been described as pathological.

DISEASES:

Huntington's Disease, Movement Disorders, Juvenile Huntington's Disease, Cadmium poisoning, Manganese Poisoning.*

* All disease associations scored above a 0.1 threshold from disgenet.com

HOMOLOGY

Human Gene HTT Alignment         
C. elegans Gene F21G4.6 align
Identity 18%
Similarity 34%
RediMODEL-KI logo sml.png

HUMANIZED C. elegans:

F21G4.6 is the homolog gene to HTT. F21G4.6 is highly expressed in germline and neuronal tissues. Partial deletion mutations in F21G4.6 have yet to demonstrate loss-of-function phenotype.  A full gene deletion mutation in F21G4.6  as HTT RediMODEL-KO is available. To create a humanized worm, the HTT gene is swapped for the F21G4.6 coding segment.   Installation of HTT in replacement of F21G4.6 remains unestablished for phenotype yet it is offered as HTT RediMODEL-KI strain. The KI strain is confirmed by PCR and shipped in the RediMODEL kit format. Included in each kit is HTT RediMODEL-KO as a reference control.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of HTT RediMODEL-KI and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for the researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KI line is sequence verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set used to verify the RediMODEL kit.

amplicon size
wild type 500 bp
HTT RediMODEL-KI 900 bp
 

Phenotype data:

Bio Assay

Conditions for the RediMODEL-KO line to yield a phenotype have not yet been determined.

strain activity
HTT RediMODEL-KO ?%
HTT RediMODEL-KI ?%

Discover HTT RediMODEL-KI Kit: | $845 each kit Buy Now

Discover RediMODEL-KI Kit: | $845 each kit

Inquire on HTT RediMODEL-KI

Please provide contact info and a company representative will contact you soon with preorder details.

HTT rediMODEL-KI inquiry

RediMODEL clinical variant in C. elegans | $6695 each kit Buy Now

HTT - huntingtin

DESCRIPTION:

Huntington's disease is a neurodegenerative disorder characterized by loss of striatal neurons due to trinucleotide expansion translating into long tracks of poly-glutamine repeat. poly-Q tracks in excess of 40 have been described as pathological.

DISEASES:

Huntington's Disease, Movement Disorders, Juvenile Huntington's Disease, Cadmium poisoning, Manganese Poisoning.*

* All disease associations scored above a 0.1 threshold from disgenet.com

HOMOLOGY

Human Gene HTT Alignment         
C. elegans Gene F21G4.6 align
Identity 18%
Similarity 34%
rediMODEL-KO

CLINICAL VARIANT C. elegans:

F21G4.6 is the homolog gene to HTT.  F21G4.6 is highly expressed in germline and neuronal tissues. Partial deletion mutations in F21G4.6 have yet to demonstrate loss-of-function phenotype. A full gene deletion mutation in F21G4.6  as HTT RediMODEL-KO is available. To create a humanized worm, HTT gene is swapped for the F21G4.6 coding segment. Installation of F21G4.6 in replacement of F21G4.6 remains unestablished for phenotype yet it is offered as HTT RediMODEL-KI control strain  A client-specified clinical variant is installed into the HTT sequence of the wt-control strain.  The result is an HTT RediMODEL-CV strain whose relative phenotypic consequence can be rapidly measured. The CV strain is confirmed by PCR and shipped in the RediMODEL kit format. Included in each kit are HTT RediMODEL-KO and HTT RediMODEL-KI as reference controls.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of HTT RediMODEL-CV and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for the researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-CV line is sequence verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set used to verify the RediMODEL kit.



amplicon size
wild type 500 bp
HTT RediMODEL-CV 900 bp
 

Phenotype data:

Bio Assay

The RediMODEL-CV line's activity is highly likely to exhibit a gain of function phenotype when installed long poly-glutamine expansions are made.

strain activity
HTT RediMODEL-KO ?%
HTT RediMODEL-CV ?%
HTT RediMODEL-KI ?%

Discover HTT RediMODEL-CV Kit: | $6695 each kit Buy Now

Discover RediMODEL-CV Kit: | $6695 each kit

Inquire on HTT RediMODEL-CV

Please provide contact info and a company representative will contact you soon with pre-order details.

HTT rediMODEL-CV inquiry

Alignment of HTT to F21G4.6

Alignment Length Identity Similarity Gaps
2096 18% 34% 27%

Let us know if you want to model clinical variants of HTT in the native F21G4.6 gene.

RediMODEL-KI native - F21G4.6 with human point mutations