RediMODEL knock out in C. elegans | $845 each kit Buy Now
SLC6A2 - norepinephrine transporter
SLC6A2 is an integral membrane protein responsible for transporting the norepinephrine neurotransmitter from synaptic space back into presynaptic neurons. (Other names: NAT1, NET, NET1, SLC6A5).
Orthostatic Intolerance, Hypertensive Disease, Mitral Valve Prolapse Syndrome, Seizures.
* All disease associations scored above a 0.1 threshold from disgenet.com
|C. elegans Gene||dat-1||align|
KNOCK OUT of C. elegans gene:
dat-1 in C. elegans is homologous to SLC6A2. The dat-1 gene functions to control dopamine levels at the synapse. When dat-1 is expressed and active, it pumps dopamine from the extracellular space and into the cytosol. Mutations in dat-1 frequently are loss-of-function alleles. The canonical allele in dat-1 is (ok157), which contains a small deletion in the gene coding region. The most potent phenotype of ok157) allele is a high rate of paralysis when exposed to hypotonic solution. To remove background mutations, the RM2702 strain is 4x outcrossed. The resulting outcrosssed ok157) allele is offered as the SLC6A2 strain in the RediMODEL kit format.
RediMODEL Kit Details:
Each kit arrives with 3 frozen tubes of SLC6A2 RediMODEL-KO and 3 tubes of N2 for control and all materials needed for 2+ days of growth.
PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.
Genotype and phenotype data provides a capacity for researcher to monitor and retain authentication, as per NIH guidelines.
The RediMODEL-KO line is verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set supplied with the RediMODEL kit.
|wild type||xxx bp|
|SLC6A2 RediMODEL-KO||xxx bp|
The RediMODEL-KO line's activity is best followed by monitoring changes in neurotransmission rates of the food intake. Pharynx pumping increases and decreases are easily monitored in ScreenChip system assay (Nemametrix.com). For Phenotype Data (pictured left), greater than 15 animals for each test condition were analyzed as young adult for a duration greater than 1 minute each.
|N2 wild type||xxx||+/- xxx|
|SLC6A2 RediMODEL-KO||xxx||+/- xxx|