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SLC6A2 - norepinephrine transporter

DISCRIPTION:

SLC6A2 is an integral membrane protein responsible for transporting the norepinephrine neurotransmitter from synaptic space back into presynaptic neurons. (Other names: NAT1, NET, NET1, SLC6A5).

DISEASES*:

Orthostatic Intolerance, Hypertensive Disease, Mitral Valve Prolapse Syndrome, Seizures.

* All disease associations scored above a 0.1 threshold from disgenet.com

Protein domain - slc6a3 dat-1.png

HOMOLOGY:

Human Gene SLC6A2 Alignment
C. elegans Gene dat-1 align
Identity 49
Similarity 67
rediMODEL-KO

KNOCK OUT of C. elegans gene:

dat-1 in C. elegans is homologous  to SLC6A2. The dat-1 gene functions to control dopamine levels at the synapse. When dat-1 is expressed and active, it pumps dopamine from the extracellular space and into the cytosol. Mutations in dat-1 frequently are loss-of-function alleles. The canonical allele in dat-1 is (ok157), which contains a small deletion in the gene coding region. The most potent phenotype of ok157) allele is a high rate of paralysis when exposed to hypotonic solution. To remove background mutations, the RM2702 strain is 4x outcrossed.  The resulting outcrosssed ok157) allele is offered as the SLC6A2 strain in the RediMODEL kit format.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of SLC6A2 RediMODEL-KO and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KO line is verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set supplied with the RediMODEL kit.


amplicon size
wild type xxx bp
SLC6A2 RediMODEL-KO xxx bp
 

Phenotype data:

phenotype activity - SLC6A4 KO.png

ScreenChip Assay

The RediMODEL-KO line's activity is best followed by monitoring changes in neurotransmission rates of the food intake. Pharynx pumping increases and decreases are easily monitored in ScreenChip system assay (Nemametrix.com). For Phenotype Data (pictured left), greater than 15 animals for each test condition were analyzed as young adult for a duration greater than 1 minute each.

strain frequency (Hz) SEM
N2 wild type xxx +/- xxx
SLC6A2 RediMODEL-KO xxx +/- xxx

Discover SLC6A2 RediMODEL-KO Kit: | $845 each kit Buy Now

Discover RediMODEL-KO Kit: | $845 each kit

Inquire on SLC6A2 RediMODEL-KO

Please provide contact info and a company representative will contact you soon with invoicing details

SLC6A2 rediMODEL-KO inquiry

Alignment of SLC6A2 to dat-1

Red asterisks indicate clinical variant alleles of pathogenic nature in humans that are conserved in C. elegans. As a result, 4 of the 12 known pathogenic alleles can be modeled in a rediMODEL-KI format. A CRISPR transgenesis approach can be used to make one of three mutations: T283R, N289S, A489T, Y548H alleles.

Lets us know if you want to model human disease in SLC6A2 with dat-1 mutations

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HTR2C - serotonin receptor

DESCRIPTION:

HTR2C is a seven-transmembrane G-protein-coupled receptor that responds to signaling through the neurotransmitter serotonin. (Other names: 5-HT1C, 5-HT2C, 5-HTR2C, 5HTR2C, HTR1C)

DISEASES*:

Weight Gain, obesity, Metabolic Syndrome, Substance Withdrawal Syndrome, hyperactive behavior.

* All disease associations scored above a 0.1 threshold from disgenet.com

prosite domain HTR2C ser-1.png

HOMOLOGY:

Human Gene HTR2C Alignment
C. elegans Gene ser-1 align
Identity 31
Similarity 48
rediMODEL-KO

C. elegans GENE:

ser-1: the homologous gene to HTR2C. The ser-1 gene function contributes to feeding behavior. When ser-1 is expressed and active, it contributes to the pumping rate observed with exogenous serotonin exposoure. Mutations in ser-1 frequently are loss-of-function alleles. The canonical allele in ser-1 is (OK345), which contains a 839 bp deletion within the gene coding region. The result is complete loss of the 4th exon and, if splicing is retained around the lost exon, the remainder of coding is 23 aa of nonsense then stop. The most easily meaured phenotype of (ok345) allele is pumping rate changes upon exposure to 5 mM serotonin. To remove background mutations, the DA1814 strain is 4x outcrossed. The resulting outcrosssed (ok345) allele is offered as the COPXXXX strain in the RediMODEL kit format.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of HTR2C RediMODEL-KO and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KO line is verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set supplied with the RediMODEL kit.


amplicon size
wild type xxx bp
HTR2C RediMODEL-KO xxx bp
 

Phenotype data:

ScreenChip Assay

The Redimodel-KO line's activity is best followed by monitoring changes in neurotransmission rates of the food intake. Pharynx pumping increases and decreases are easily monitored in ScreenChip system assay (Nemametrix.com). For Phenotype Data (pictured left), greater than 15 animals for each test condition were analyzed as young adult for a duration greater than 1 minute each.

strain frequency (Hz) SEM
N2 wild type xxx +/- xxx
HTR2C RediMODEL-KO xxxx +/- xxx

Discover HTR2C RediMODEL-KO Kit: | $845 each kit Buy Now

Discover RediMODEL-KO Kit: | $845 each kit

Inquire on HTR2C RediMODEL-KO

Please provide contact info and a company representative will contact you soon with invoicing details

HTR2C rediMODEL-KO inquiry

Alignment of HTR2C to ser-1

Grey asterisks indicate clinical variant alleles of pathogenic nature in humans but they are not conserved in C. elegans. Modeling human mutations will require insertion of human cDNA of HTR2C into the native locus of ser-1

Lets us know if you want to model human disease in HTR2C with ser-1 mutations

RediMODEL knock out in C. elegans | $845 each kit Buy Now

HTR2B - serotonin transporter

DISCRIPTION:

HTR2B is a seven-transmembrane G-protein-coupled receptor that responds to signaling through the neurotransmitter serotonin. (Other names: 5-HT(2B), 5-HT-2B, 5-HT2B

DISEASES*:

Heart failure, Heart valve disease, Cardiomegaly, Pulmonary Hypertension, Hypertensive disease, Adverse reaction to drug.

* All disease associations scored above a 0.1 threshold from disgenet.com

Protein domain - slc6a3 dat-1.png

HOMOLOGY:

Human Gene HTR2B Alignment
C. elegans Gene ser-1 align
Identity 29
Similarity 48
rediMODEL-KO

C. elegans GENE:

ser-1: A homologous gene to HTR2B. The ser-1 gene function contributes to feeding behavior. When ser-1 is expressed and active, it contributes to the pumping rate observed with exogenous serotonin exposoure. Mutations in ser-1 frequently are loss-of-function alleles. The canonical allele in ser-1 is (ok345), which contains a 839 bp deletion within the gene coding region. The result is complete loss of the 4th exon and, if splicing is retained around the lost exon, the remainder of coding is 23 aa of nonsense then stop. The most easily measured phenotype of (ok345) allele is pumping rate changes upon exposure to 5 mM serotonin. To remove background mutations, the DA1814 strain is 4x outcrossed. The resulting outcrosssed (ok345) allele is offered as the HTR2B RediMODEL-KO strain. The strain is confirmed by PCR and shipped in the RediMODEL kit format.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of HTR2B RediMODEL-KO and 3 tubes of N2 for control and all materials needed for 2+ days of growth. PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KO line is verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set supplied with the RediMODEL kit.


amplicon size
wild type xxx bp
HTR2B RediMODEL-KO xxx bp
 

Phenotype data:

ScreenChip Assay

The RediMODEL-KO line's activity is best followed by monitoring changes in neurotransmission rates of the food intake. Pharynx pumping increases and decreases are easily monitored in ScreenChip system assay (Nemametrix.com). For Phenotype Data (pictured left), greater than 15 animals for each test condition were analyzed as young adult for a duration greater than 1 minute each.

strain frequency (Hz) SEM
N2 wild type xxx +/- xxx
HTR2B RediMODEL-KO xxxx +/- xxx

Discover HTR2B RediMODEL-KO Kit: | $845 each kit Buy Now

Discover RediMODEL-KO Kit: | $845 each kit

Inquire on HTR2B RediMODEL-KO

Please provide contact info and a company representative will contact you soon with invoicing details

HTR2B rediMODEL-KO inquiry

Alignment of HTR2B to ser-1

Red asterisks indicate clinical variant alleles of pathogenic nature in humans that are conserved in C. elegans. As a result, 3 of the 6 known pathogenic alleles can be modeled in a rediMODEL-KI format. A CRISPR transgenesis approach can be used to make one of three mutations: G51E, F173L, R393P alleles.

Lets us know if you want to model human disease in HTR2B with ser-1 mutations