RediMODEL transgenics in C. elegans

HTR2A - serotonin receptor

GENE DESCRIPTION:

  • Function: HTR2A is a GPCR involved in synaptic neurotransmission response.
  • Disease: Genetic defects in HTR2a have been found to be highly associated with Schizophrenia and bipolar disorder.
 

Products:

RediMODEL Kit -  a series of humanized animal models specifically engineered for easy measurement of phenotype defects caused by clinically-relevant disease mutations.   

rediMODEL-KO

HTR2A RediMODEL KO: Generated in the C. elegans homolog ser-1 with a small deletion of coding sequence to create a knock-out (KO) strain. Loss of function from ser-1 deletion leads to hyperactivity and increased pharynx pumping rate. Major phenotype readout is the observation of pharyngeal pumping rate. An outcrossed version of the small deletion is currently available for preorder as gene knock-out.

 

HTRA2A RediMODEL KI: Generated in the C. elegans homolog ser-1 with human gene insertion to create a knock-in (KI) strain. Entire coding of ser-1 is replaced with wild-type HTR2A coding sequence. An HTR2A gene replacement is available for preorder as a humanized wt line.

 

HTR2A RediMODEL CV: Generated in the C. elegans homolog ser-1 with human gene insertion containing a clinical variant allele to create a clinical-variant (CV) strain. Entire coding of ser-1 is replaced with HTR2A coding sequence containing an amino-acid change polymorphism that has been observed in patient populations. Upon request, an HTR2A line with client-specific polymorphism is made as a clinical variant allele.

 

RediMODEL knock-out in C. elegans | $845 each kit Buy Now

HTR2A - serotonin receptor

DESCRIPTION:

The 5-hydroxytryptamine receptor 2A is a GPCR involved in neurotransmission response to release of serotonin at the synapse.

DISEASES:

Schizophrenia, Bipolar Disorder, Hyperalgesia, Major Depressive Disorder, Chronic Alcoholic Intoxication, Anorexia Nervosa, Autistic Disorder, Obsessive-Compulsive Disorder, Psychotic Disorders, Obesity, Migraine Disorders, Chronic Obstructive Airway Disorder, Dyskinetic syndrome, Metabolic Diseases, Fever, Basal Ganglia Diseases, Hyperactive behavior, Cocaine-Related Disorders.

All disease associations scored above a 0.1 threshold from disgenet.com

Homology domain - HTR2A ser-1.png

HOMOLOGY:

Human Gene HTR2A Alignment         
C. elegans Gene ser-1 align
Identity 27%
Similarity 47%

KNOCK-OUT of C. elegans gene:

ser-1 is a homolog gene to HTR2A.The ser-1 gene function contributes to feeding behavior. When ser-1 is expressed and active, it contributes to the pumping rate observed with exogenous serotonin exposure. Mutations in ser-1 frequently are loss-of-function alleles. The canonical allele in ser-1 is (ok345), which contains an 839 bp deletion within the gene coding region and is thought to render a knock-out (KO) of gene function. The most easily measured phenotype for the KO is an increased pharynx pumping phenotype. To decrease the risk of background mutations, the KO strain is 4x outcrossed. The resulting outcrossed strain containing (ok345) allele is offered as the HTR2A RediMODEL-KO strain. The KO strain is confirmed by PCR and shipped in the RediMODEL kit format.  Included in the kit is N2 wild-type strain as a reference control.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of HTR2A RediMODEL-KO and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for the researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KO line is sequence verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set used to verify the RediMODEL kit.



amplicon size
wild type 500 bp
HTR2A RediMODEL-KO 900 bp
 

Phenotype data:

Bio Assay

The RediMODEL-KO line's activity is best followed by monitoring changes in neurotransmission rates of food intake. Pharynx pumping increases and decreases are easily monitored in the ScreenChip system assay (Nemametrix.com). Using the Low Stimulation Pharynx Pumping Protocol (P001), a significant difference in pumping rate is observed.

Low Stimulation Pharynx Pumping Protocol (P001)


strain wt frequency (Hz)
N2 wild type 10%
HTR2A RediMODEL-KO 100%

Discover HTR2A RediMODEL-KO Kit: | $845 each kit Buy Now

Discover RediMODEL-KO Kit: | $845 each kit

Inquire on HTR2A RediMODEL-KO

Please provide contact info and a company representative will contact you soon with preorder invoicing details.

HTR2A rediMODEL-KO inquiry

RediMODEL knock-in of human gene replacement in C. elegans | $845 each kit Buy Now

HTR2A - serotonin receptor

DESCRIPTION:

The 5-hydroxytryptamine receptor 2A is a GPCR involved in neurotransmission response to release of serotonin at the synapse.

DISEASES:

Schizophrenia, Bipolar Disorder, Hyperalgesia, Major Depressive Disorder, Chronic Alcoholic Intoxication, Anorexia Nervosa, Autistic Disorder, Obsessive-Compulsive Disorder, Psychotic Disorders, Obesity, Migraine Disorders, Chronic Obstructive Airway Disorder, Dyskinetic syndrome, Metabolic Diseases, Fever, Basal Ganglia Diseases, Hyperactive behavior, Cocaine-Related Disorders.

All disease associations scored above a 0.1 threshold from disgenet.com

Protein domain - slc6a3 dat-1.png

HOMOLOGY:

Human Gene HTR2A Alignment         
C. elegans Gene ser-1 align
Identity 27%
Similarity 47%
RediMODEL-KI logo sml.png

HUMANIZED C. elegans:

ser-1 is a homolog gene to HTR2A.The ser-1 gene function contributes to feeding behavior. When ser-1 is expressed and active, it contributes to the pumping rate observed with exogenous serotonin exposure. Deletion mutations in ser-1  as HTR2A RediMODEL-KO causes a loss-of-function phenotype as increased rate of pumping. To create a humanized worm,  HTR2A gene is swapped for the ser-1 coding segment. Installation of HTR2A in replacement of ser-1 causes restoration of normal pumping phenotype and is offered as HTR2A RediMODEL-KI. The KI strain is confirmed by PCR and shipped in the RediMODEL kit format. Included in the kit is HTR2A RediMODEL-KO as a reference control.

genomic locus - HTR2A CV.png

RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of HTR2A RediMODEL-KI and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KI line is verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set supplied with the RediMODEL kit.



amplicon size
wild type 500 bp
HTR2A RediMODEL-KI 900 bp
 

Phenotype data:

Bio Assay

The RediMODEL-KI line's activity is best followed by monitoring changes in neurotransmission rates of the food intake. Pharynx pumping increases and decreases are easily monitored in ScreenChip system assay (Nemametrix.com). using the Low Stimulation Pharynx Pumping Protocol (P001), a significant difference in pumping rate is observed.

Low Stimulation Pharynx Pumping Protocol (P001)

strain wt frequency (Hz)
HTR2A RediMODEL-KO 100%
HTR2A RediMODEL-KI 10%

Discover HTR2A RediMODEL-KI Kit: | $845 each kit Buy Now

Discover RediMODEL-KI Kit: | $845 each kit

Inquire on HTR2A RediMODEL-KI

Please provide contact info and a company representative will contact you soon with preorder details.

HTR2A rediMODEL-KI inquiry

RediMODEL clinical variant in C. elegans | $6695 each kit Buy Now

HTR2A - serotonin receptor

DESCRIPTION:

The 5-hydroxytryptamine receptor 2A is a GPCR involved in neurotransmission response to release of serotonin at the synapse.

DISEASES:

Schizophrenia, Bipolar Disorder, Hyperalgesia, Major Depressive Disorder, Chronic Alcoholic Intoxication, Anorexia Nervosa, Autistic Disorder, Obsessive-Compulsive Disorder, Psychotic Disorders, Obesity, Migraine Disorders, Chronic Obstructive Airway Disorder, Dyskinetic syndrome, Metabolic Diseases, Fever, Basal Ganglia Diseases, Hyperactive behavior, Cocaine-Related Disorders.

All disease associations scored above a 0.1 threshold from disgenet.com

Protein domain - slc6a3 dat-1.png

HOMOLOGY:

Human Gene HTR2A Alignment         
C. elegans Gene ser-1 align
Identity 27%
Similarity 47%
rediMODEL-KO

CLINICAL VARIANT C. elegans:

ser-1 is a homolog gene to HTR2A.The ser-1 gene function contributes to feeding behavior. When ser-1 is expressed and active, it contributes to the pumping rate observed with exogenous serotonin exposure. Deletion mutations in ser-1  as HTR2A RediMODEL-KO causes a loss-of-function phenotype as increased rate of pumping.  Installation of HTR2A in replacement of ser-1 results in the HTR2A RediMODEL-KI control strain and cause restoration of normal pharynx pumping phenotype.  A client-specified clinical variant is installed into the HTR2A sequence of the wt-control strain.  The result is an HTR2A RediMODEL-CV strain whose relative phenotypic consequence can be rapidly measured. The CV strain is confirmed by PCR and shipped in the RediMODEL kit format. Included in the kit are HTR2A RediMODEL-KO and HTR2A RediMODEL-KI as reference controls.


RediMODEL Kit Details:

Each kit arrives with 3 frozen tubes of HTR2A RediMODEL-CV and 3 tubes of N2 for control and all materials needed for 2+ days of growth.

PLEASE PUT FROZEN TUBES AT -80C UPON ARRIVAL.

Authentication:

Genotype and phenotype data provides a capacity for the researcher to monitor and retain authentication, as per NIH guidelines.

Genotype data:

PCR Assay

The RediMODEL-KI line is sequence verified and followed by PCR. The gel image in Genotype Data shows the expected PCR band pattern in an agarose gel for the primer set used to verify the RediMODEL kit.



amplicon size
wild type 500 bp
HTR2A RediMODEL-KI 900 bp
 

Phenotype data:

phenotype activity - KOCVKI inv.png

Bio Assay

The RediMODEL-CV line's activity is best followed by monitoring changes in neurotransmission rates of food intake. Pharynx pumping increases and decreases are easily monitored in the ScreenChip system assay (Nemametrix.com). Using the Low Stimulation Pharynx Pumping Protocol (P001), a significant difference in pumping rate is observed.

Low Stimulation Pharynx Pumping Protocol (P001)

strain frequency (Hz)
HTR2A RediMODEL-KO 100%
HTR2A RediMODEL-CV ??%
HTR2A RediMODEL-KI 10%

Discover HTR2A RediMODEL-CV Kit: | $6695 each kit Buy Now

Discover RediMODEL-CV Kit: | $6695 each kit

Inquire on HTR2A RediMODEL-CV

Please provide contact info and a company representative will contact you soon with design and invoicing details.

HTR2A rediMODEL-CV inquiry

Alignment of HTR2A to ser-1

Alignment Length Identity Similarity Gaps
473 27% 47% 20%

Lets us know if you want to model clinical variants of HTRA2A in the native ser-1 gene.

RediMODEL-KInative - ser-1 with human point mutations