Tol2 Transgenics

Tol2-mediated gene Insertion

Tol2 is a Zebrafish technology used to insert a transgene into the genome. mRNA for the transposase is injected along with the desired transgene. The transposase creates a genomic double-strand break and the transgene is inserted via homologous recombination.


Tol2 insertion

Donor homology plasmid used for large cargo insertion in the zebrafish genome. Timelines: RediMIX + Injection - 3 week; Full-Build - 6 month.

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