Zebrafish CRISPR Services

Knudra specializes in custom CRISPR services in Zebrafish. We have designed and delivers over 1,500 transgenic lines to meet the research needs of our clients. Below are several types of CRISPR services we offer. 

GFP Tagging - other tag inserted

A Tagging CRISPR knock-in (KI) project is designed to insert either a fluorescent tag (typically GFP or mCherry), or to insert a pull-down tag (typically 3xFLAG, HA, or TAP). Timelines: RediMIX + Injection - 3 week; Full-Build - 6 month.

quote for Tagging

 

PointMUTATION - Swap of amino acid content

A pointMUTATION CRISPR knock-in (KI) project is designed to change an amino aicd or small region in a gene. Timelines: RediMIX + Injection - 3 week; Full-Build - 6 month.

quote for pointMUTATION

 

ExonDEL - Exon deletion

An exonDEL CRISPR knock-out (KO) project is designed to delete a section of a coding sequence of any desired gene. Typically a conserved exon, or group of exons, is deleted. Timelines: RediMIX + Injection - 3 week; Full-Build - 6 month.

quote for exonDEL

 

3frameSTOP - early stop codon inserted

A 3frameSTOP CRISPR project is designed to insert 3frameSTOP cassette early in a gene's coding sequence. The early stop codon from a 3 frame STOP insertion frequently creates a full knock-out (KI) allele. Timelines: RediMIX + Injection - 3 week; Full-Build - 6 month.

quote for 3frameSTOP

 

RandomINDEL - Non-specific mutant generation

A randomINDEL CRISPR project is designed to randomly insert or delete content. No donor homology is used. Highly efficient but imprecise indel project that may or may not results in a Knock-out (KO).

quote for randomINDEL

 

Unsure? - Contact us

email Knudra

CRISPR - exonDEL

 

CRISPR project is designed to for precision deletion of a segment of a gene. 

 
zebrafish CRISPR exonDEL

CRISPR - 3frameSTOP

 

CRISPR project is designed to insert a 3 frame stop early in your desired gene. 

 
zebrafish CRISPR 3frameSTOP

CRISPR - Tagging

 

CRISPR project is designed to tag either termini of your desired gene.  Tagging examples are GFP mCherry, 3xFLAG, HA, TAP, etc. 

 
zebrafish CRISPR Tagging

CRISPR - randomINDEL

 

CRISPR project is designed to generate random mutations occurring frequently as small indels

 
zebrafish CRISPR randomINDEL

CRISPR - pointMUTATION

 

CRISPR project is designed to change a amino acid or other small region of coding. 

 
zebrafish CRISPR pointMUTATION

Zebrafish Transgenics Question

 

Let us know if you need more information about how our transgenesis methods can fit your needs.

 
Zebrafish Transgenics Question

Tol2 Transgenics

Tol2-mediated gene Insertion

Tol2 is a Zebrafish technology used to insert a transgene into the genome. mRNA for the transposase is injected along with the desired transgene. The transposase creates a genomic double-strand break and the transgene is inserted via homologous recombination.

 
 

Tol2 insertion

Donor homology plasmid used for large cargo insertion in the zebrafish genome. Timelines: RediMIX + Injection - 3 week; Full-Build - 6 month.

quote for Tol2 transgencs

Tol2 transgenics

 

Please feel out the form below with any details you might have on your project.

 
Tol 2 transgenics request