C. elegans RecombiREAD Reporter

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RecombiREAD Reagent. Experiments in C elegans demonstrate recombiREAD reagent can act as co-CRISPR tool that acts within 24 hrs of injection.  

RecombiREAD-RED Reagent designed to turn red in C. elegans embryo after injection. The recombiREAD reagent has significant utility act as co-CRISPR tool that acts within 24 hrs of injection.

Efficiency of RecombiREAD in finding jackpot plates.  A) configuration of recombiREAD reporter recRED. B) embryo's reporting activation of homologous recombination as induced red fluorescence at 24hrs post injection. C) efficiency of recombiREAD for finding injections containing targeted gene edit.

Efficiency of RecombiREAD in finding jackpot plates.  A) configuration of recombiREAD reporter recRED. B) embryo's reporting activation of homologous recombination as induced red fluorescence at 24hrs post injection. C) efficiency of recombiREAD for finding injections containing targeted gene edit.

Using a RecombiREAD reporter: "reporter RED", examination of embryos at 24 hrs for high levels of red embryos provides indication the remaining cohort has high likelihood of containing a desired edit. For example, using the  RecombiREAD reporter reporter RED as a co-CRISPR reagent to detect dpy-10 edits as roll-positive animals, 76% of plates marked by presence of red embryos had desired CRISPR edit. Contrast to a plasmid contain a RFP driven by constitutive promoter, only 21% of of marked plates containing desired edit.  Without selection marker, less than 10% of all plates had desired edit. 

Currently RecombiREAD reporter reporter RED is available for evaluation. Kits of RecombiREAD are planned to be available in additional fluorophore markers. 

 

RecombiREAD reporters:

 RR100001 - Reporter RED - 10 ul - 200 ng/ul - (please inquire for price)

 RR100001 - Reporter RED - 10 ul - 200 ng/ul - (please inquire for price)

 RR100002 - Reporter GREEN - 10 ul - 200 ng/ul - (in development)

 RR100002 - Reporter GREEN - 10 ul - 200 ng/ul - (in development)

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